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ifitm1  (Proteintech)


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    Proteintech ifitm1
    Ifitm1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ifitm1/product/Proteintech
    Average 93 stars, based on 35 article reviews
    ifitm1 - by Bioz Stars, 2026-03
    93/100 stars

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    RF levels in the overexpression HEK293T cell lines. ( A ). Quantification of RF mRNA expression in the overexpressing cell lines and control cells (Ctl) by qRT-PCR. Because of their very similar sequences (93,3% identity), IFITM2 and IFITM3 transcript levels were measured with the same primer pair. The results correspond to the means and standard deviations of two RNA extractions, each of them measured in duplicates. ( B ). Expression of IFITM proteins in the overexpressing cell lines detected by western blot. <t>IFITM1</t> (Top) and IFITM2/3 (Bottom) were tested independently in all 6 cell lines and were detected only in the corresponding overexpressing cell lines (apparent MW for both 17 kDa, top panels). Total protein content in each well was checked by looking at actin content (MW 46 kDa, lower panels).
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    RF levels in the overexpression HEK293T cell lines. ( A ). Quantification of RF mRNA expression in the overexpressing cell lines and control cells (Ctl) by qRT-PCR. Because of their very similar sequences (93,3% identity), IFITM2 and IFITM3 transcript levels were measured with the same primer pair. The results correspond to the means and standard deviations of two RNA extractions, each of them measured in duplicates. ( B ). Expression of IFITM proteins in the overexpressing cell lines detected by western blot. <t>IFITM1</t> (Top) and IFITM2/3 (Bottom) were tested independently in all 6 cell lines and were detected only in the corresponding overexpressing cell lines (apparent MW for both 17 kDa, top panels). Total protein content in each well was checked by looking at actin content (MW 46 kDa, lower panels).
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    RF levels in the overexpression HEK293T cell lines. ( A ). Quantification of RF mRNA expression in the overexpressing cell lines and control cells (Ctl) by qRT-PCR. Because of their very similar sequences (93,3% identity), IFITM2 and IFITM3 transcript levels were measured with the same primer pair. The results correspond to the means and standard deviations of two RNA extractions, each of them measured in duplicates. ( B ). Expression of IFITM proteins in the overexpressing cell lines detected by western blot. IFITM1 (Top) and IFITM2/3 (Bottom) were tested independently in all 6 cell lines and were detected only in the corresponding overexpressing cell lines (apparent MW for both 17 kDa, top panels). Total protein content in each well was checked by looking at actin content (MW 46 kDa, lower panels).

    Journal: Scientific Reports

    Article Title: Combined effects of restriction factors and transduction adjuvants on lentiviral vector gene transfer efficacy

    doi: 10.1038/s41598-025-22470-9

    Figure Lengend Snippet: RF levels in the overexpression HEK293T cell lines. ( A ). Quantification of RF mRNA expression in the overexpressing cell lines and control cells (Ctl) by qRT-PCR. Because of their very similar sequences (93,3% identity), IFITM2 and IFITM3 transcript levels were measured with the same primer pair. The results correspond to the means and standard deviations of two RNA extractions, each of them measured in duplicates. ( B ). Expression of IFITM proteins in the overexpressing cell lines detected by western blot. IFITM1 (Top) and IFITM2/3 (Bottom) were tested independently in all 6 cell lines and were detected only in the corresponding overexpressing cell lines (apparent MW for both 17 kDa, top panels). Total protein content in each well was checked by looking at actin content (MW 46 kDa, lower panels).

    Article Snippet: Membranes were blotted with anti IFITM1 antibody (Proteintech 60,074–1-Ig, 1/5000) or anti IFITM2/3 antibody (Proteintech 66,081–1-Ig, 1/5000).

    Techniques: Over Expression, Expressing, Control, Quantitative RT-PCR, Western Blot